Role and Scope of Cell Line Authentication DNA Test in India
The commercialization of DNA profiling tests and the technological advancements in the field have made getting a DNA test in India possible and affordable. Besides identification profiling and relationship tests, DNA testing is also used for Cell Line Authentication.
A Cell Line Authentication Test helps find out the genetic and other errors in the cells under study/experimentation.
What is a Cell Line Authentication Test Done For?
In an experiment, using the right cells and thus their proper identification is critical. A cell line being studied needs to match with the reference cell line. This purpose can be achieved using a Cell Line Authentication DNA Test.
With the help of a Cell Line Authentication Test, one can identify the following types of errors in the cells being studied.
Misidentification at Source
A misidentified cell line at the source is a common occurrence. If unaware, one may proceed with experimenting or studying the same (wrong group of) cells. There have been cases where researchers kept working on a misidentified cell line, getting unintended results every time for years, and ultimately realizing the actual error. Such errors can be avoided by getting a Cell Line Authentication Test.
Cross-Contamination
Cross-contamination between two or more cell lines generally arises within the lab environment. In such cases, cells from other mediums can alter the identity of the cell, which can be easily detected using a Cell Line Authentication DNA Test in India. Other causes of cross-contamination include mislabelling, keeping multiple cell lines within the same safety cabinet, and using the same tools to handle them.
Genetic Drift
While in the lab environment, the cells can interact with the environment and diverge with the passage of time. This is deteriorating for the experiment, as the cells may be completely changed in their identity.
More often, it is necessary that these errors are checked for in a study or experiment with the help of an accurate and reliable Cell Line Authentication test.
Process of Cell Line Authentication DNA Test in India
Following are the cases when a Cell Line Authentication services in India is required,
- Collecting the cell line to be investigated
- Extraction of the DNA from the cell
- Analyzing the DNA’s polymorphism STR regions
- PCR amplification of the polymorphic sites on the DNA
- Capillary Electrophoresis of the resulting products of PCR (Polymerase Chain Reaction)
- Creation profile of the repeating regions
When Should a Cell Line Authentication Test be Done?
Following are the cases when a Cell Line Authentication test is required,
- when a new cell line is established, thereby eradicating the possibility of misidentification of the cell line at the source
- before freezing, at any time when the cell line under study is inconsistent, reducing the chances of random genetic drifts
- when more than one cell line is being handled in a laboratory, saving the culture (and cells) from cross contamination
- before conducting new experiments
- before applying for grants and publishing about the experiment in journals, etc.
Applications of Cell Line Authentication DNA Tests in India
There are a variety of applications of a Cell Line Authentication Test.
One of its prime applications includes drug and vaccine development. Before the medicines and the vaccines reach the shelves of pharmacies and are ultimately used by people, they must undergo rigorous trial procedures to assess the drug/vaccine response, the body’s response to it, possible side effects, and like information.
Another commonplace for cell line authentication tests are educational institutions in genetics study. Cell Line Authentication DNA Test in India finds huge scope among students and independent researchers, as well.
Best Place to Get a Cell Line Authentication DNA Test in India
One may find an accredited lab to get a Cell Line Authentication DNA Test in India. However, it’s best to get it from a certified lab/company.
DNA Forensics Laboratory Pvt. Ltd. is among the best and most trusted DNA testing companies in India, providing DNA tests for various purposes, including paternity, maternity, siblingship, immigration, organ transplant DNA tests, as well as Cell Line Authentication tests.
DFL has been providing accredited peace of mind DNA tests for relationship identification and is also trusted by Indian courts and the government. Moreover, we are the only company providing Legal DNA Tests for Indian courts of law. People looking for the best place to get accurate and reliable reports will find DNA Forensics Laboratory to be an ideal choice for a Cell Line Authentication DNA Test in India.
