Looking for a Magnetic Beads based reagent for post-PCR clean-up?

BySam Walton
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If you are looking for a paramagnetic beads-based reagent for manual and automated post-PCR and post-enzymatic reaction clean-up, the HighPrep™ PCR Clean-up System allows efficient purification of DNA fragments and tight size-specific selection of amplicons.

This PCR clean-up system is a paramagnetic beads-based post-PCR clean-up reagent. It is added to the reaction mixture. The DNA fragments selectively bind to the paramagnetic bead particles. The purification consists of the removal of salts, primers, primer-dimers, dNTPs. The highly purified DNA is then eluted with a low salt elution buffer or water. The purified DNA can now be used for downstream applications, such as –

  • library construction
  • next generation sequencing (NGS)
  • Sanger sequencing
  • Cloning
  • restriction digestions
  • adapter ligation
  • microarrays

This protocol can be used for manual setups. The kit can also be adapted to automatic liquid handling instruments. It can be stored for 14 months when stored as directed.

The HighPrep™ PCR Clean-up reagent is perfectly suited for amplicon size selection, and NGS library preparation for sequencing as well as for post-PCR clean-up of shorter amplicons or restriction enzyme digestion products.

Applications of HighPrep™ PCR Clean-up System

The HighPrep™ PCR Clean-up System can be used for post-PCR and post-enzymatic reactions during:

  • NGS library preparation
  • Amplicon size selection for NGS
  • Microarrays
  • PCR
  • Restriction enzyme digestions, adapter ligations
  • Cloning
Benefits of HighPrep™ PCR Clean-up System compared to alternatives
  • Rapid and reliable post-PCR and post-enzymatic reaction clean-up
  • Efficient wash from contaminants
  • High recovery of amplicons >100bp
  • Uniform fragments size distribution
  • Adaptable to high throughput liquid handling workstations

Check out the HighPrep™ PCR Clean-up System as a direct replacement to AMPure at MagBio Genomics at (301) 302-0144. The HighPrep™ PCR Clean-up System is a magnetic beads-based reagent for manual and automated post-PCR and post-enzymatic reaction clean-up, for NGS library clean-up and reliable amplicon size selection.